TREM2 shapes myeloid cell activations in trauma- and autoimmune-mediated neurodegeneration

TREM2 signaling in myeloid cells functions as a major pathology-induced immune signaling hub in trauma- and autoimmune-mediated neurodegeneration. Here we compared the influence of TREM2 signaling on single-cell transcriptional profiles of mouse myeloid populations during the pathologies of spinal cord injury (SCI) and multiple sclerosis (MS) models. Single-cell RNA sequencing of myeloid cells in spinal cords from Wt and Trem2-/- mice under naive, SCI (1wk after injury) and experimental autoimmune encephalomyelitis (EAE, peak stage) conditions was performed using BD Rhapsody system. Myeloid cells were enriched using CD11b positive selection from at least two animals in each sample. Different single-cell suspension samples were mixed together using BD Single-cell Multiplexing Kits for high sample throughput and batch effects elimination purposes.