Levels of Polyamines in Feces of Laying Hens Fed with Agave Fructans (Agave Tequilana, Weber) in Association with the Quality and Production of Egg

ABSTRACT In this study two experiments were carried out, the effect of a diet supplemented with fructans of Agave tequilana Weber, was analyzed in Hy-line hens in regards to their egg quality and production as well as on the levels of the polyamines, putrescine, spermidine and spermine in their feces. In the first study 300 Hy-line W-36 hens per group, which were aged 36 weeks, were randomly separated as follows for each of the three treatments. One group of 100 hens was fed with a diet supplemented with 0.1% fructans. Another group of 100 hens was supplemented with 0.2% fructans and a group of 100 hens without any fructans was added as a control group. Feed consumption was lower in the supplemented groups compared to the control group (p<0.05). Egg yolk quality was measured using Haugh units. The quality of the shell was studied using an Egg Force reader (g pressure/mm2). Putrescine levels was measured in 10 animals in each group. In the second study, 1,155 laying hens, aged one day from the Hy-line W-35 genetic line were also randomly divided into three groups containing 385 hens in each one (A, B, C). Egg laying levels and weight was measured during 35 weeks. The egg laying percentage increased considerably in hens ingesting supplemented diets (p<0.05) and the egg weight was greater mainly in the supplementation with 0.1% fructans (p<0.05). Thus, it was shown that diet supplementation with fructans of agave improves egg quality and homeostasis and food consumption in the Hy-line hen.

摘要 本研究开展两项实验，探究添加龙舌兰（Agave tequilana Weber）果聚糖的日粮对海兰（Hy-line）蛋鸡的蛋品质、产蛋性能，以及其粪便中腐胺、亚精胺、精胺三种多胺水平的影响。
第一项实验中，36周龄的海兰W-36（Hy-line W-36）蛋鸡每组300只，随机分为3个处理组：其中1组100只饲喂添加0.1%果聚糖的日粮，另1组100只饲喂添加0.2%果聚糖的日粮，剩余100只不添加果聚糖作为对照组。结果显示，果聚糖添加组的采食量显著低于对照组（p<0.05）。蛋黄品质采用哈氏单位（Haugh units）进行测定，蛋壳品质通过蛋壳强度测定仪（Egg Force reader，单位：g压力/mm²）进行分析。每组选取10只蛋鸡，测定其粪便中的腐胺水平。
第二项实验中，选取1日龄的海兰W-35（Hy-line W-35）品系产蛋鸡共1155只，随机分为A、B、C三组，每组385只。实验周期为35周，期间测定产蛋率与蛋重。结果表明，饲喂果聚糖添加日粮的蛋鸡产蛋率显著提升（p<0.05），且饲喂0.1%果聚糖组的蛋重增幅最为显著（p<0.05）。
综上，本研究证实，添加龙舌兰果聚糖的日粮可改善海兰蛋鸡的蛋品质、机体内稳态及采食量。