Raw data, assembly and annotation results for: “Ultra-deep sequencing enables high-fidelity recovery of biodiversity for bulk arthropod samples without PCR amplification”

The following data comes from the study Ultra-deep sequencing enables high-fidelity recovery of biodiversity for bulk arthropod samples without PCR amplification, which is the first systematic meta-barcoding study sequencing the total DNA from insect communities independent of PCR amplification. Following mitochondrial enrichment using differential centrifugation, a preliminary sample and a formal sample are achieved respectively with 2.2G and 13.2G from the Illumina HiSeq 2000 platform using 100 bp paired-end (PE) sequencing, following manufacturers instructions. The data was assembled using SOAPdenovo2 and annotated using the MT_annotation_BGI pipeline (tools and pipelines available here: doi:10.5524/100046). Further details are available in the published paper and the metadata provided in the MIMS formatted table.

本数据集源自研究论文《超深度测序无需聚合酶链式反应（Polymerase Chain Reaction，PCR）扩增即可高保真恢复节肢动物混合样本的生物多样性》，该研究为首项无需PCR扩增、直接对昆虫群落总DNA进行测序的系统性元条形码（meta-barcoding）研究。通过差速离心法完成线粒体富集后，分别获得预实验样本与正式样本，二者在因美纳（Illumina）HiSeq 2000测序平台上采用100 bp双端（PE）测序，产出数据量分别为2.2G与13.2G，测序流程严格遵循厂商说明书操作。本数据集使用SOAPdenovo2进行序列组装，并通过MT_annotation_BGI分析流程完成基因注释（相关工具与流程可通过doi:10.5524/100046获取）。更多详细信息可查阅已发表论文及MIMS格式元数据表中附带的元数据。