RNA-seq of Arabidopsis thaliana seedlings growing axenically, or in the presence of different bacterial Synthetic Community treatments that induced different root morphology

This study evaluated the transcriptomic profiles of Arabidopsis thaliana (Col-0) plants grown along four SynCom treatments that induced differential primary root growth. Treatments Dropout Variovorax and DropoutVariovoraxBurkholderia induced primary root growth inhibition (RGI), while treatments Full and DropoutBurkholderia mantained a stereotypical long primary root. All seeds were surface-sterilized with 70% bleach, 0.2% Tween-20 for 8 min, and rinsed three times with sterile distilled water to eliminate any seed-borne microbes on the seed surface. Seeds were stratified at 4 °C in the dark for two days. Plants were germinated on vertical square 10 X 10 cm agar plates with JM containing 0.5% sucrose, for 7 days. Then, 10 plants were transferred to each of the bacteria-inoculated agar plates containg JM without sucrose RNA-seq of Arabidopsis thaliana seedlings growing axenically, or in the presence of different bacterial Synthetic Community treatments that induced different root morphology