Fig2E_18S-gel_R2_LG378.sgd

HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with or without a 99bp sequence from the STOML2 gene inserted. RNA was extracted and examined by northern blotting. Ethidium bromide-stained gel images are also included to show rRNA.

将HEK293T ishXrn1细胞经多西环素（doxycycline）处理3~4天以诱导Xrn1基因敲低，随后转染带有或不带有插入自STOML2基因的99bp序列的荧光素酶报告基因（luciferase reporter）。提取RNA后通过Northern印迹杂交（Northern blotting）进行检测，同时附带溴化乙锭（ethidium bromide）染色的凝胶图像以展示核糖体RNA（ribosomal RNA，rRNA）。