CUT&RUN analyses of fetal Leydig cells and testicular interstitial cells

Ad4BP/SF-1 is a transcription factor regulating gene expression related to steroidogenesis and energy metabolism. Previous studies have indicated that Ad4BP/SF-1 regulates fetal Leydig cell differentiation from progenitor cells. However, the underlying regulatory mechanisms remain unclear. Here, we reveal that Ad4BP/SF-1 directly regulates energy metabolisms in differentiated fetal Leydig cells through transcriptional upregulation. Ad4BP/SF-1 profiling of fetal Leydig cells (S-EGFP cells) and testicular interstitial cells (W-EGFP cells) was performed using CUT&RUN.