Functional annotation of the phage vB_EcoP_Z06 gene

Firstly, the nucleic acid extraction and library construction of bacteriophage vB_EcoP_Z06 were carried out, and sequencing was performed using DNBSEQ-T7. To ensure the reliability of subsequent information analysis results, we used fastp [1] to filter and quality control the obtained raw sequencing data, cut adapters, remove low-quality reads and reads with high N content, and obtain clean reads. Use metaSPAdes software to perform de novo assembly on clean reads, while selecting different k-mer lengths for testing and obtaining the best assembly results. Then, use BWA software to align clean reads to the assembled genome sequence for statistical coverage. Finally, the genes were functionally annotated using eggNOG mapper.