eYFP inserted in antisense orientation within a silenced genomic region in Trypanosoma brucei
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http://flowrepository.org/id/FR-FCM-Z55D
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资源简介：
RBP33 depletion results in the appearance of novel transcripts that seem to be properly processed and are therefore potentially translatable in Trypanosoma brucei. This was directly addressed by inserting a gene encoding enhanced yellow-fluorescent protein (eYFP) in an antisense orientation at two different cSSR loci in bloodstream trypanosomes. The resulting cell lines were further transfected with plasmid pGR70 for tetracycline-inducible RNAi of RBP33, and eYFP expression was measured using flow-cytometry.
Conclusion:
eYFP expression increased 3 to 10-fold upon depletion of RBP33 in T. brucei cell lines.
创建时间：
2022-06-01
