Gibberella zeae. Fusarium graminearum PH-1

The genetic map of Gibberella zeae lineage 7 was constructed using a cross between the type strain PH-1 (NRRL 31084) and a strain from Minnesota, 00-676 (NRRL 34097). Cytological examination of parental strains indicated that each have four chromosomes. A total of 112 random ascospore progeny were analyzed for 122 genetic loci; 110 of the markers exhibited Mendelian inheritance. Genetic markers consist of single nucleotide polymorphisms (n=66), RFLPs (n= 29), and microsatellites (n=27). Most SNPs and RFLPs were identified by sequencing alleles derived from EST loci for both parental strains; others were based on sequences at the ends of smaller scaffolds. One EST-based marker (HK235) and a marker based on the major ribosomal RNA repeat were not represented in the assembly but both map to linkage group 9 on chromosome 4. Microsatellites were obtained from a genome-wide scan for simple tandem repeats by the method of Benson (Nucleic Acids Res. 27:573-580, 1999) and then used for polymorphism screening at selected loci.