Large scale production of melanocyte derived from pluripotent stem cells for biotherapeutic approaches to treat skin pigmentation disorders. Large scale production of melanocyte derived from pluripotent stem cells for biotherapeutic approaches to treat skin pigmentation disorders

In this study, we successfully defined a highly efficient standardize method to derive hPSCs into a pure population of functional melanocytes. These cells can be maintain for more than 20 passages without observed any senescence. Transcriptomic anlaysis revealed a differentiation process with a logic gene expression profile following the kinetic of melanocyte embryonic development as previously described in mice. Our findings represent a fruitful ground that can serve as a database tool to decipher molecular signatures in normal and pathological conditions Overall design: Kinetic of a differentiation process from D0 to D30, comparaison of P0 and P4 stages to established a maturation phase and caracterisation of mature Mel-hESC in comparaison with hESC and HEM.