Effect of Tramesan on murine cell lines of melanoma (B16).

Murine cell lines of melanoma (B16) were treated with 0.38 μM Tramesan or untreated (control). The amount of melanin was calculated by spectrophotometer (Abs405), normalised for untreated samples and the percentage of its increase was evaluated and compared to untreated samples. Cell growth was calculated by counting the number of viable cells. The results are the mean ± SD of 5 separate experiments Relative expression of the Nrf-2 gene normalised on untreated cells was calculated using the 2-ΔΔCt method.