Single-cell transcriptome analysis reveals distinct cell populations in the celiac ganglia

In the peripheral nervous system, the prevertebral ganglion (PVG) serves as an important relay station, transmitting centrifugal signals to visceral organs; the PVG is also known to innervate enteroanal afferent neurons (IFANs) and spinal sensory neurons innervating the intestinal tract. It has been suggested that there are neural circuits within the PVG consisting of sensory and sympathetic neurons. Here, we used a single nuclei RNA sequencing method to characterize the gene expression profiles of individual cells constituting the ventral ganglion in normal rats. These data provide valuable material for examining the neural circuits within the PVG. Overall design: We performed a single-nuclei RNA-seq experiment on the celiac ganglion from 10 normal male Sprague-Dawley rats.