Video 5. Cell death of Mm infected macrophages showing rapid signal disappearance morphologies.

Double transgenic Tg(mpeg1:EGFP) and Tg(lyz:DsRed2) larvae infected with Mm (red) were imaged alive every ~60 sec using CLSM (Nikon A1). The GFP-positive (green) macrophage infected with Mm (red) indicated by white arrow undergoes cell death showing rapid signal disappearance at 54 minutes and immediate recruitment of another macrophage. The maximum intensity projection from 11 CLSM images (step size in z-direction, 5.56 µm) is shown at 10 frames per second. Scale bar: 20 µm.

使用共聚焦激光扫描显微镜（Confocal Laser Scanning Microscopy，CLSM；尼康A1系统）以约每60秒一次的频率，对感染了红色荧光标记Mm的双转基因Tg(mpeg1:EGFP)与Tg(lyz:DsRed2)斑马鱼幼鱼进行活体成像。由白色箭头标注的、被红色Mm感染的增强型绿色荧光蛋白（Enhanced Green Fluorescent Protein, EGFP）阳性（绿色）巨噬细胞发生细胞死亡，表现为54分钟时荧光信号快速消散，并随即招募了另一个巨噬细胞。本演示视频以每秒10帧的速率展示了11幅CLSM图像的最大强度投影图（z轴方向步进间距为5.56 µm）。比例尺：20 µm。