SUCROSE TRANSPORTER GENES FOR INCREASING PLANT SEED LIPIDS

This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation， including for example， DGAT， Lecl and ODPl transcription factor. In specific embodiments， overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to a high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters. This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation， including for example， DGAT， Lecl and ODPl transcription factor. In specific embodiments， overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to a high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.

本发明涉及编码SUT2或SUT4蔗糖转运蛋白基因的多核苷酸序列。本发明披露了提升植株种子含油量以及评估植株种子含油量提升幅度的方法。本发明所公开的组合物与方法采用了多种编码蔗糖转运蛋白的序列，以及多种影响脂肪酸积累的序列，例如DGAT、Lecl与ODPl转录因子。在特定实施例中，相较于仅携带未过表达SUT2或SUT4转运蛋白的重组DNA构建体的高油植株，同时过表达SUT2和/或SUT4蔗糖转运蛋白与DGAT基因可进一步提升植株种子的油脂产量。本发明涉及编码SUT2或SUT4蔗糖转运蛋白基因的多核苷酸序列。本发明披露了提升植株种子含油量以及评估植株种子含油量提升幅度的方法。本发明所公开的组合物与方法采用了多种编码蔗糖转运蛋白的序列，以及多种影响脂肪酸积累的序列，例如DGAT、Lecl与ODPl转录因子。在特定实施例中，相较于仅携带未过表达SUT2或SUT4转运蛋白的重组DNA构建体的高油植株，同时过表达SUT2和/或SUT4蔗糖转运蛋白与DGAT基因可进一步提升植株种子的油脂产量。