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Immunofluorescence staining images for seqFISH and seqFISH+ datasets

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Zenodo2021-03-15 更新2026-05-25 收录
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TIFF images: Brain1 (seqFISH): ~1,500 cells Brain2 (seqFISH): ~1,500 cells SeqFISH+. ~500 cells Each field of view (FOV) has two channels, Dapi and Nissl. Each field of view is a TIFF file. The files: Brain1.tar.gz, Brain2.tar.gz, seqfishplus.images.tar.gz are the raw TIFF images. The files: segmented.*.tar.gz are the segmented images (in PDF and SVG format). Segmentation is shown as yellow outlines. Please cite the following papers: Qian Zhu, Sheel Shah, Ruben Dries, Long Cai, Guo-Cheng Yuan Identification of spatially associated subpopulations by combining scRNAseq and sequential fluorescence <em>in situ</em> hybridization data Nature biotechnology, 36, 1183-1190 (2018) Chee-Huat Linus Eng, Michael Lawson, Qian Zhu, Ruben Dries, Noushin Koulena, Yodai Takei, Jina Yun, Christopher Cronin, Christoph Karp, Guo-Cheng Yuan, Long Cai Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH Nature, 2019, 568(7751):235-239

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2021-02-15
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