Fu yulian

The dataset includes Western blot, ROS, and IC50 data. Each experiment was conducted using SCC25 and CAL27 cell lines, with three technical replicates and three biological replicates per experiment. For Western Bolt experiments, cells underwent specific treatments followed by protein extraction. Quantification was performed via BVA (70V-30min, 100V-90min), after which exposure was conducted. Image software measured the gray values of each band, yielding relative expression levels as target protein gray value/reference protein gray value.
ROS: Cells were seeded in 96-well plates. After confluence, reagents and drugs were added. Data were measured in a fluorometer after 24 hours. Final relative ROS levels were calculated using the method described in the XLSX table, including blank controls, negative controls, and experimental groups. Calculation method: Experimental group OD value - average value of blank wells to eliminate systematic error. Data is then normalized (corrected experimental group OD value / average value of negative control group) to obtain relative ROS levels.
IC50: Similarly, cells were seeded in 96-well plates. After attachment, cisplatin at varying concentrations was added. After 24 hours, OD values were measured in the microplate reader for the blank wells, negative control group, and experimental group.
Calculation method: Same as the ROS calculation method.