Rat Cerebellum RNAseq based on dietary fatty acids.

Dietary n-3 polyunsaturated fatty acids (PUFA) influence postnatal brain growth and development. However, little is known about the impacts of dietary n-3 PUFA in juvenile animals post weaning, a time of rapid growth. The purpose of the study was to determine what genes within the juvenile cerebellar transcriptome are responsive to dietary n-3 PUFA deprivation. Three week old male Sprague-Dawley rats (n=10/group) were fed diets consisting of either α-linolenic acid (ALA; 18:3n-3) sufficient soybean oil (SO) or ALA-deficient, corn oil (CO) for four weeks. Next-generation sequencing was performed on the cerebellar tissue to identify differentially expressed genes.

膳食中的n-3多不饱和脂肪酸（PUFA）对出生后大脑的生长与发展具有显著影响。然而，关于断奶后青少年动物膳食n-3 PUFA的影响知之甚少，而此阶段正处于快速生长期。本研究旨在确定青少年小脑转录组中哪些基因对膳食n-3 PUFA的缺乏具有响应性。实验中使用了10只/组的3周龄雄性Sprague-Dawley大鼠，饲养了四周，其饮食分别为富含α-亚麻酸（ALA；18:3n-3）的足量大豆油（SO）或缺乏ALA的玉米油（CO）。随后，对小脑组织进行了下一代测序，以识别差异表达基因。