Supplemental Data: Variations in mineral prestrain, nanostructure, and microarchitecture play a role in intervertebral disc loading

Data related to the publication "Multimodal imaging reveals multiscale mechanical interplay in vertebral endplate microarchitecture during intervertebral disc loading", Parmenter et al., 2024, https://www.biorxiv.org/content/10.1101/2024.08.19.608559v2Full methodology described in the above publication.Total data size = 41 GB<b>Data used for statistical analysis:</b>sCT_DVC_stats_data.csv <i>: Data used for statistical analysis shown in Fig. 3 and Fig. 4</i>DIAD_stats_data.csv <i>: Data used for statistical analysis shown in Fig. 6</i><b>WAXD processing code:</b>processing_folder_D-period.py <i>: python code for measuring diffraction peak parameters</i>ctMapperGUI.py <i>: python code for mapping WAXD parameters onto X-ray projection image</i><br><b>Example sCT and DVC dataset (sample 5):</b>162365_2560xy_2160z_pag100_us2p5_8bit.tif<i> : reconstructed sCT image of sample under 1 N preload, 13 GB, recommend opening in imageJ</i>162366_2560xy_2160z_pag100_us2p5_8bit.tif <i>: reconstructed sCT image of sample after 40 micron displacement, 13 GB, recommend opening in imageJ</i>162365_caudal_mindist4p5_mesh.inp <i>: mesh of caudal VEP used for creating the DVC pointcloud, ABAQUS input file</i>162366_cranial_mindist4p5_mesh.inp <i>: mesh of cranial VEP used for creating the DVC pointcloud, ABAQUS input file</i>162365_caudal_pointcloud.txt <i>: caudal VEP pointcloud for DVC</i>162365_cranial_pointcloud.txt <i>: cranial VEP pointcloud for DVC</i>dvc_162365_caudal.disp <i>: DVC displacement results for caudal VEP, output from iDVC executable, can visualise in Matlab</i>dvc_162365_cranial.disp <i>: DVC displacement results for cranial VEP, </i><i>output from iDVC executable, </i><i>can visualise in Matlab</i>dvc_162365_caudal_1-sw25.Lstr.csv <i>: strain data for caudal endplate generated from disp file</i>sample5_caudal_step1.csv <i>: strain data for caudal endplate</i>sample5_caudal_step1.csv <i>: strain data for cranial endplate generated from disp file</i>sample5_force_readout.xlsx <i>: force, displacement, time data readout from the mechanical testing rig during the experiment</i>sample5BinaryROIs.zip <i>: binary images of regions used for microarchitecture analysis</i>sample5ROImsvalues.xlsx <i>: microarchitecture values for regions of interest</i><b>Example correlative sCT and WAXD dataset (sample 16):</b>17565_2560xy_2160z_us1_8bit.tif <i>: reconstructed sCT image, 13 GB, recommend opening in imageJ</i>17565_BinaryROI_tifs <i>: binary image regions used for microarchitecture analysis</i>17567 002 dperiod map.png <i>: 002 d-period mapped on X-ray projection</i>17567 002 width map.png <i>: 002 peak width mapped on X-ray projection</i>17567_310_Peak_D-period_data.txt <i>: 310 d-period for each beampath through the sample</i>17567_310_Peak_width_data.txt <i>: 310 peak width for each beampath through the sample</i>17567_D-period_data_002.txt <i>: 002 d-period for each beampath through the sample</i>17567_Width_data_002.txt <i>: 002 peak width for each beampath through the sample</i>k11-17567.nxs <i>: raw diffraction data (part 1)</i><i>, recommend opening in DAWN (both parts required)</i>k11-17567.zip <i>: raw diffraction data (part 2), recommend opening in DAWN </i><i>(both parts required)</i>k11-17567_processed_220722_184414.nxs <i>: processed diffraction data, recommend opening in DAWN</i><br>

本数据集关联论文《多模态成像揭示椎间盘载荷过程中椎体终板微结构的多尺度力学相互作用》（英文原名：Multimodal imaging reveals multiscale mechanical interplay in vertebral endplate microarchitecture during intervertebral disc loading），作者为Parmenter等人，发表于2024年，原文链接：https://www.biorxiv.org/content/10.1101/2024.08.19.608559v1。完整实验方法已在上述论文中详述。数据集总容量为41 GB。<br><b>用于统计分析的数据：</b><br>sCT_DVC_stats_data.csv <i>：用于生成图3与图4的统计分析数据</i><br>DIAD_stats_data.csv <i>：用于生成图6的统计分析数据</i><br><b>广角X射线衍射（Wide-Angle X-ray Diffraction, WAXD）处理代码：</b><br>processing_folder_D-period.py <i>：用于提取衍射峰参数的Python代码</i><br>ctMapperGUI.py <i>：用于将WAXD参数映射至X射线投影图像的Python代码</i><br><br><b>示例结构化计算机断层扫描（structured CT, sCT）与数字体相关（Digital Volume Correlation, DVC）数据集（样本5）：</b><br>162365_2560xy_2160z_pag100_us2p5_8bit.tif <i>：样本在1 N预载荷下的重建sCT图像，容量为13 GB，推荐使用ImageJ打开</i><br>162366_2560xy_2160z_pag100_us2p5_8bit.tif <i>：样本经过40微米位移后的重建sCT图像，容量为13 GB，推荐使用ImageJ打开</i><br>162365_caudal_mindist4p5_mesh.inp <i>：用于生成DVC点云的尾侧椎体终板（vertebral endplate, VEP）网格文件，为ABAQUS输入文件</i><br>162366_cranial_mindist4p5_mesh.inp <i>：用于生成DVC点云的头侧VEP网格文件，为ABAQUS输入文件</i><br>162365_caudal_pointcloud.txt <i>：用于DVC分析的尾侧VEP点云数据</i><br>162365_cranial_pointcloud.txt <i>：用于DVC分析的头侧VEP点云数据</i><br>dvc_162365_caudal.disp <i>：尾侧VEP的DVC位移结果文件，由iDVC可执行程序生成，可在Matlab中进行可视化</i><br>dvc_162365_cranial.disp <i>：头侧VEP的DVC位移结果文件，由iDVC可执行程序生成，可在Matlab中进行可视化</i><br>sample5_caudal_step1.csv <i>：由位移文件生成的尾侧椎体终板应变数据</i><br>sample5_cranial_step1.csv <i>：由位移文件生成的头侧椎体终板应变数据</i><br>sample5_force_readout.xlsx <i>：实验过程中由力学测试台采集的力、位移与时间数据</i><br>sample5BinaryROIs.zip <i>：用于微结构分析的感兴趣区域二值图像压缩包</i><br>sample5ROImsvalues.xlsx <i>：各感兴趣区域的微结构参数数据</i><br><br><b>关联式sCT与WAXD示例数据集（样本16）：</b><br>17565_2560xy_2160z_us1_8bit.tif <i>：重建的sCT图像，容量为13 GB，推荐使用ImageJ打开</i><br>17565_BinaryROI_tifs <i>：用于微结构分析的感兴趣区域二值图像集</i><br>17567 002 dperiod map.png <i>：映射至X射线投影图像的002晶面d间距图</i><br>17567 002 width map.png <i>：映射至X射线投影图像的002峰宽图</i><br>17567_310_Peak_D-period_data.txt <i>：样本各入射束路径对应的310晶面d间距数据</i><br>17567_310_Peak_width_data.txt <i>：样本各入射束路径对应的310峰宽数据</i><br>17567_D-period_data_002.txt <i>：样本各入射束路径对应的002晶面d间距数据</i><br>17567_Width_data_002.txt <i>：样本各入射束路径对应的002峰宽数据</i><br>k11-17567.nxs <i>：原始衍射数据（第1部分），推荐使用DAWN软件打开（需同时使用两部分数据）</i><br>k11-17567.zip <i>：原始衍射数据（第2部分），推荐使用DAWN软件打开（需同时使用两部分数据）</i><br>k11-17567_processed_220722_184414.nxs <i>：处理后的衍射数据，推荐使用DAWN软件打开</i><br>