Multigenerational effects of graphene oxide nanoparticles on Acheta domesticus DNA stability

The main goal of this project is elucidation and description of mechanisms of decreased survival (in the first and second generations), and decreased DNA stability (in subsequent generations) after constant exposure of cricket cohorts (Acheta domesticus -model organism) to sublethal amounts of GO in their food, and also evaluation of physiological and biochemical symptoms of adjustment to such stressful condition.The partgoals of this project are to check and describe:1. connections between exposure of A. domesticus to GO and composition of gut microbiota and, in turn, their physiology (growth, reproduction and general fitness);2. microbiomes composition in GO-exposed crickets in relation to strain and generation;3. microbiome metabolic profiles in GO-exposed crick -dependent scheme;4. energy budget indices in both strains of GO-exposed crickets in relation to exposure time; digestive enzymes activity;5. general cell state in GO-exposed crickets in relation to strain and exposure time;6. effects of GO on telomere length, telomerase activity, DNA methylation and DNA damage and repair level in successive generations of two house cricket strains

本项目的核心目标为阐明并描述家蟋蟀（Acheta domesticus，模式生物（model organism））种群持续暴露于食物中致死剂量以下的氧化石墨烯（GO）后，其第一、二代存活率下降以及后续世代DNA稳定性降低的机制，并评估此类生物适应此种胁迫环境的生理与生化表征。本项目的子目标包括检验并描述以下内容：
1. 家蟋蟀暴露于GO与肠道微生物群（gut microbiota）组成之间的关联，以及该关联反过来对其生理状态（生长、繁殖及综合适合度（general fitness））的影响；
2. 暴露于GO的蟋蟀肠道微生物群组成与其品系及世代的相关性；
3. 暴露于GO的蟋蟀肠道微生物群代谢谱的品系依赖性规律；
4. 两种GO暴露蟋蟀品系的能量收支指数随暴露时长的变化情况，以及消化酶活性；
5. 暴露于GO的蟋蟀整体细胞状态与其品系及暴露时长的相关性；
6. GO对两种家蟋蟀品系连续世代的端粒长度（telomere length）、端粒酶活性（telomerase activity）、DNA甲基化（DNA methylation）以及DNA损伤与修复（DNA damage and repair）水平的影响。