ASBT inhibition alleviates cholangiopathy and hepatic fibrosis development in Cyp2c70-/- mice

RNA-seq was used to compare the liver gene differentiation between WT and Cyp2c70-/- mice fed with chow and SC-435 containing diet. We aimed to characterize the cholestatic liver phenotype in the Cyp2c70 -/- mouse and test whether ASBT inhibition reduced injury. Examine liver gene expression between WT and Cyp2c70-/- mice fed with chow and SC-435 containing diet.本研究采用RNA测序（RNA-seq）技术，对比野生型（WT）与Cyp2c70纯合敲除（Cyp2c70-/-）小鼠在喂食普通饲料与添加SC-435饲料时的肝脏基因表达差异。本研究旨在明确Cyp2c70-/-小鼠的胆汁淤积性肝脏表型，并验证ASBT（顶端钠依赖性胆汁酸转运蛋白，Apical Sodium-dependent Bile Acid Transporter）抑制是否可减轻肝脏损伤。本研究同时检测了喂食普通饲料与添加SC-435饲料的野生型与Cyp2c70纯合敲除小鼠的肝脏基因表达水平。