Melanisation occurs in embryos injected with fzd3a, 9b, 10-MO at 1 dpf.

Notes.Numbers in parenthesis are of significantly melanised embryos. We did not inject 5 ng/embryo or more of fzd10 MO with other morpholinos because we found combinatorial injection of 10 ng of fzd3a-MO and 5 ng of fzd10-MO caused severe necrosis (data not shown). Here, “severe” necrosis means we found necrotic cell death everywhere in the body, whilst “slight” means necrosis was observed mainly in the head. Most embryos show elongated body axis, and those are categorised into “elongated axis”; others, categorised as “shortened axis”, lack tail and sometimes have severely bent or reduced trunk.