Integrative Analysis Reveals Shegan-Mahuang Decoction Modulates Immunometabolism and Inflammatory Response in Asthma [scRNA-seq]

SMD alleviated lung injury in asthmatic mice with a significant reduction in leukocytes and recovered inflammatory marker levels. Mice treated with SMD showed higher microbial diversity and reversed metabolic imbalances compared to the asthma group. SMD acted primarily by modulating gene expression enriched in inflammatory processes in the lungs. Analysis of intercellular interactions revealed changes in the interaction network between different cell types during SMD treatment. Particularly, macrophages and T cells showed diversity in differentiation, suggesting the role of cellular heterogeneity and their interactions in immune response to asthma. ELISA results showed that SMD reduced asthma-induced inflammatory factors and IgE levels. Overall design: After 7 days of acclimatization, mice were randomly assigned to four experimental groups containing 15 mice in each group: control, model, SMD and Prednisone (PDN) groups.Lungs tissues from model and SMD groups were taken for scRNA-seq.