DNA metabarcoding of foliar fungal communities in an untreated vineyard

This sequence dataset (FASTQ format) was obtained by metabarcoding fungal communities of 20-days old leaves in an untreated vineyard located in INRA Vilenave d'Ornon (France). The ribosomal internal transcribed spacer region 1 (ITS1) was amplified with the ITS1F / ITS2 primer pair and sequenced on an Illumina MiSeq platform (v2 chemistry, 500 cycles). "M" samples were collected from leaves harboring symptoms of powdery mildew (Erysiphe necator), while "S" samples were collected from visually healthy leaves. "ZI" samples correspond to disease spots and "ZS" samples were taken from the healthy leaf blade of infected leaves. The "info_16S" caption is misleading, the sequences are fungal ITS sequences.

本序列数据集采用FASTQ格式（FASTQ），通过对法国INRA维勒纳夫多尔农（INRA Vilenave d'Ornon）一处未施药葡萄园中的20日龄叶片的真菌群落开展元条形码测序（metabarcoding）获得。研究以ITS1F/ITS2引物对扩增核糖体内部转录间隔区1（ribosomal internal transcribed spacer region 1，ITS1），并在Illumina MiSeq测序平台（采用v2化学试剂，500个循环）上完成测序。其中，M样本采自带有白粉病（Erysiphe necator）症状的叶片，S样本采自外观健康的叶片；ZI样本对应病斑区域，ZS样本采自染病叶片的健康叶肉组织。标注为"info_16S"的说明文字存在误导性，实际序列为真菌ITS序列。