SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in April 2012

Flow cytometry data was collected in April 2012, in waters off South Australia.

The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.

Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

流式细胞术（Flow Cytometry）数据采集于2012年4月，采集区域为澳大利亚南部近海海域。

本研究的总体目标为：建立澳大利亚南部大陆架生态系统的基础背景资料，阐明上升流与盐度流出事件对微生物群落的影响，最终构建该区域的生物地球化学模型。采样工作依托RV Ngerin号科考船执行的航次开展，该航次属于南澳大利亚综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）研究项目的一部分。每个航次中，研究人员均对叶绿素荧光最大值（Deep Chlorophyll Maximum, DCM）层的物理、化学及生物学特性展开了调查。本数据集涵盖超微型浮游植物、细菌与病毒的流式细胞术检测数据。

本次研究共设置6个主要采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S, 136.278°E）、B2（35.418°S, 136.148°E）、B3（35.258°S, 136.048°E）、SAM2CP/B4（35.168°S, 135.418°E）及SAM5CB/B5（35.008°S, 135.198°E）；剩余1个为离岸站位B1（36.188°S, 136.178°E），地处袋鼠岛西南海域。

结合站位间距（14–25海里）、陆架中层海流的平均流速（0.01 m·s⁻¹）以及科考船的平均航速（9节），可判定各站位采集的水团互不相同。此外，研究期间还会偶尔从袋鼠岛国家参考站位（National Reference Station, NRS，35.832°S, 136.447°E），以及发生盐度流出事件的南澳大利亚斯宾塞湾湾口锚系站（SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S, 136.690°E）采集额外样本。