Use of pro-biotics in Striped Trumpeter culture

This data represents research conducted as part of a PhD project on Striped Trumpeter (Latris lineata).Knowledge of the microbial bacterial community of the larvae, seawater and live feeds is necessary in order to develop an understanding of what represents a healthy microbial ecology relevant for larval fish rearing success. 16S ribosomal RNA (rRNA) gene-based clone library and terminal restriction fragment length polymorphism (TRFLP) analyses were utilized to examine the microbial community associated with larvae cultured under different “greenwater” conditions and determine the success of probiont application in live feeds used for larval fish rearing. Though water quality was apparently influential on larval survival, no specific bacterial species present either on the larvae or in surrounding tank water could be attributed to reduced survival. Two bacterial strains, Pseudoalteromonas sp. ST8 and Vibrio sp. ST7 were found to inhibit bacteria pathogenic to larval fish through production of extracellular substances. Strain ST8 was found to be compatible with artemia, rotifers and larval fish and outcompeted the fish pathogen Vibrio proteolyticus in competition experiments. Encapsulation experiments indicated strain ST8 was transferrable to artemia and thus represent a potential probiont for larval fish mariculture.

本数据集源自一项针对条纹笛鲷（Striped Trumpeter，Latris lineata）的博士研究项目。为厘清与仔鱼繁育成功相关的健康微生物生态特征，亟需掌握仔鱼、海水及鲜活饵料中的细菌群落组成信息。本研究采用基于16S核糖体RNA（rRNA）基因的克隆文库及末端限制性片段长度多态性（TRFLP）分析技术，对不同“绿水”养殖条件下培育的仔鱼相关微生物群落进行解析，并评估有益菌（probiont）在仔鱼繁育鲜活饵料中的施用效果。尽管水质对仔鱼存活率具有显著影响，但并未发现仔鱼体表或养殖池水体中的特定细菌类群与存活率降低存在关联。研究筛选得到两株细菌：假交替单胞菌属（Pseudoalteromonas）菌株ST8与弧菌属（Vibrio）菌株ST7，二者可通过分泌胞外物质抑制对仔鱼具有致病性的细菌。实验证实菌株ST8可与卤虫、轮虫及仔鱼兼容，且在竞争试验中相较于鱼类致病菌溶蛋白弧菌（Vibrio proteolyticus）更具竞争优势。包埋试验结果显示，菌株ST8可成功定植于卤虫体内，因此具备成为仔鱼海水繁育用有益菌的应用潜力。