Genotype and raw sequencing data for "Development of a high-density sub-species-specific targeted SNP assay for Rocky Mountain bighorn sheep (Ovis canadensis canadensis)"

We designed a novel SNP (Single nucleotide polymorphism) genotyping assay for use with Tecan's Allegro genotyping technology (a form of single primer enrichment technology or SPET) for Rocky Mountain bighorn sheep. Our overall goal was to develop an assay which targeted 50,000 SNPs in this species but during development we designed a trial 10,000 SNP assay. We trialled both the 10,000 and 50,000 SNP assay on 40 Rocky Mountain bighorn sheep, eight individuals from two other subspecies of bighorn sheep, and eight individuals from two other species of thinhorn sheep. The Tecan library prep was performed on these samples and then sequenced on an Illumina NextSeq next generation sequencer. Data here contains raw sequencing reads for the 56 individuals genotyped using the 10,000 SNP and 50,000 SNP assays, and the VCF genotype files produced when aligning and SNP calling was performed on these reads.

本研究针对落基山大角羊，开发了一款适配Tecan Allegro基因分型技术（一种单引物富集技术（single primer enrichment technology, SPET））的新型单核苷酸多态性（Single nucleotide polymorphism, SNP）基因分型检测试剂盒。本研究的总体目标是开发靶向该物种50000个SNP位点的检测试剂盒，但研发期间先期开发了一款包含10000个SNP位点的试验性检测试剂盒。我们使用这两款试剂盒，分别对40只落基山大角羊、2个其他大角羊亚种的8只个体，以及2个其他细角羊物种的8只个体开展了基因分型试验。对上述样本完成Tecan文库制备流程后，使用Illumina NextSeq下一代测序仪进行测序。本数据集包含使用10000 SNP和50000 SNP分型试剂盒完成基因分型的56只个体的原始测序读段，以及基于这些读段进行序列比对与SNP位点识别后得到的VCF基因型文件。