Gene expression profile of 22RV1 prostate cancer cells upon SPINK1 knockdown

Serine Peptidase Inhibitor, Kazal type 1 (SPINK1) overexpression represents the second-largest prostate cancer (PCa) subtype associated with increased risk of biochemical recurrence and poor prognosis. To determine the pathways regulated by SPINK1 in 22RV1 prostate cancer cells, we performed shRNA mediated knockdown of SPINK1 using lentiviral constructs. Scrambled shRNA was used as a control. pGIPZ constructs against SPINK1 (shSPINK1-1, shSPINK1-2, shSPINK1-3) and control shScrambled construct were purchased from Dharmacon. Global gene expression profile of 22RV1 shSPINK1 cells compared to 22RV1 shScrambled control cell. Two color Experiment, Organism: Homo sapiens, Agilent's Human Whole Genome 8x60k GE Microarray (AMADID-72363).