Ypt1 IPs

Yeast strains expressing either GST-tagged or untagged Ypt1 were grown in either normal media or in media treated with 10mM DTT, to induce UPR A replicate experimental design type is where a series of replicates are performed to evaluate reproducibility or as a pilot study to determine the appropriate number of replicates for a subsequent experiments表达谷胱甘肽S-转移酶标签（GST-tagged）融合蛋白或无标签Ypt1的酵母菌株，分别于正常培养基或经10毫摩尔二硫苏糖醇（DTT）处理的培养基中培养，以诱导非折叠蛋白反应（Unfolded Protein Response，UPR）。此类重复实验设计指通过开展一系列重复实验以评估实验可重复性，或作为预实验以确定后续实验所需的适宜重复次数。