Data Sheet 1_Geographical variation of Ceracris kiangsu gut microbiota and its association with environmental factors.docx

The yellow-spined bamboo locust (Ceracris kiangsu) is one of the most destructive forest pests in China, causing severe damage to bamboo forests across multiple provinces. Understanding the relationship between its gut microbiota and environmental factors is crucial for revealing its ecological adaptability and migration potential. This study used 16S rRNA high-throughput sequencing to analyze gut bacterial communities of C. kiangsu from six geographically distinct populations in China. The results showed that Firmicutes and Proteobacteria were the dominant phyla, while bacterial diversity and composition varied significantly among populations. Precipitation and sunshine duration were identified as the main environmental factors most strongly associated with microbial community structure. These findings suggest that environmental conditions are strongly associated with variation in the gut microbiota of C. kiangsu, potentially affecting its adaptability and outbreak dynamics. This research provides new insights into the ecological mechanisms underlying pest distribution and offers a microbiome-based foundation for developing sustainable control strategies to reduce the agricultural and forestry losses caused by this species.