Collagen, fibrinogen and thrombin biological addesive is effective in treating experimental liver injuries

ABSTRACT Objective : to evaluate the effectiveness of a collagen-based adhesive associated with fibrinogen and thrombin in experimental liver injury in rats. Methods : the study included 30 Wistar rats randomly divided into three groups: A, B and C. All underwent standard liver traumatic injury. In group A the lesion was treated with the adhesive; in group B, with conventional absorbable suture; and in group C, there was no treatment. We analyzed the time of hemostasis, mortality, occurrence of adhesions and any histological changes. Results : there was no statistical difference in relation to mortality (p = 0.5820). The group treated with the adhesive showed the lowest hemostasis times (p = 0.0573, odds ratio 13.5) and lower incidence of adhesions (p = 0.0119). Microscopic histological alterations of Groups A and B were similar, with foreign body granuloma formation separating the adhesive material or the suture from the hepatic stroma. Conclusion : the adhesive of collagen associated with fibrinogen and thrombin was effective in the treatment of experimental hepatic injury, providing a lower incidence of adhesions between the liver and surrounding structures.

摘要
研究目的：评估结合纤维蛋白原（fibrinogen）与凝血酶（thrombin）的胶原蛋白基粘合剂（collagen-based adhesive）在大鼠实验性肝损伤中的治疗有效性。
研究方法：本研究纳入30只Wistar大鼠，随机分为A、B、C三组。所有大鼠均接受标准肝脏创伤造模。A组对损伤部位采用该粘合剂进行处理；B组采用常规可吸收缝合线进行处理；C组不予任何治疗。本研究分析了止血时间、死亡率、粘连发生情况及各项组织学变化。
研究结果：三组死亡率无统计学差异（p=0.5820）。粘合剂处理组的止血时间最短（p=0.0573，优势比13.5），且粘连发生率更低（p=0.0119）。A组与B组的镜下组织学改变相似，均可见异物肉芽肿形成，将粘合剂或缝合线与肝间质分隔开来。
研究结论：结合纤维蛋白原与凝血酶的胶原蛋白基粘合剂可有效治疗实验性肝损伤，能够降低肝脏与周围组织结构间的粘连发生率。