CaneToad_West_vs_East_Differential_expression_results

Differential gene expression results in invasive Cane toad muscle tissue between the Western Australia range edge (West) and the Queensland range core (East). Results produced in edgeR from associated RSEM individual expression count files (See attached archive). In edgeR testing was conducted using the classic approach for two groups, CPM-filtered counts were TMM-normalised, quantile-adjusted conditional maximum likelihood method was used to estimate dispersions and the exact test was used to call differentially expressed genes and compute p-values. Columns are: transcript ID, Log Fold-change between the regions (-ve values donate down-regulation in the West and +ve the opposite), Log CPM (counts per million mapped reads), P-value of differential expression, FDR is False discovery rate.

本数据集聚焦入侵性蔗蟾（Cane toad）的肌肉组织，涵盖西澳大利亚分布边界（下称"西部"）与昆士兰州分布核心区（下称"东部"）两组样本的差异基因表达分析结果。该结果依托关联的RSEM个体表达计数文件，通过edgeR软件分析生成（详见附带归档文件）。edgeR分析采用两组样本经典分析范式：先对经CPM过滤的计数数据实施TMM标准化，通过分位数调整的条件极大似然法估计离散度，再借助精确检验鉴定差异表达基因并计算p值。结果文件各列依次为：转录本ID（transcript ID）、两组区域间的对数倍数变化（负值表示西部样本中该基因表达下调，正值则反之）、对数CPM（每百万比对读段计数）、差异表达的p值、FDR（错误发现率，False Discovery Rate）。