Chromosomal-level genome assembly of the reef gardener long-spine urchin, Diadema setosum

The long-spined sea urchin Diadema setosum is an algal and coral feeder widely distributed in the Indo-Pacific and can cause severe bioerosion on the reef community.<b> </b>Nevertheless, the lack of genomic information has hindered the study its ecology and evolution. Here, we report the chromosomal-level genome (885.8 Mb) of the long-spined sea urchin D. setosum using a combination of PacBio long-read sequencing and Omni-C scaffolding technology. The assembled genome contained scaffold N50 length of 38.3 Mb, 98.1 % of BUSCO (Geno, metazoa_odb10) genes, and with 98.6% of the sequences anchored to 22 pseudo-molecules/chromosomes. A total of 27,478 genes including 23,030protein-coding genes were annotated. The high-quality genome of D. setosum presented here provides a significant resource for further understanding on the ecological and evolutionary studies of this coral reef associated sea urchin.

长棘海胆（Diadema setosum）是广泛分布于印度-太平洋海域的藻类与珊瑚食性生物，可对珊瑚礁群落造成严重的生物侵蚀。然而，基因组信息的缺失阻碍了其生态学与进化学研究。本研究结合PacBio长读长测序与Omni-C支架技术，报道了长棘海胆D. setosum的染色体级基因组（885.8 Mb）。组装获得的基因组支架N50长度达38.3 Mb，完整度覆盖98.1%的BUSCO（Geno, metazoa_odb10）基因集，且98.6%的序列锚定至22条伪分子/染色体。本研究共注释得到27478个基因，其中包含23030个蛋白质编码基因。本次报道的高质量长棘海胆基因组，将为深入理解该珊瑚礁关联海胆的生态学与进化学研究提供重要资源。