Functions of histone crotonylation in early mouse embryos by RNA-seq

Echs1 plays important roles in histone lysine crotonylation. Although it has been studied in many systems, its functions in early embryos remain unclear. In this study, we knocked down Echs1 in mouse oocytes and examined its impact on transcriptome in early mouse embryos. Mouse zygotes were treated with sodium acetate or sodium crotonate, and early 2-cell embryos were collected for RNA-seq analysis.