Leveraging hyperspectral imaging to identify drought tolerant Populus species and genotypes within species

The aim of this study was to identity variation in drought tolerance across genotypes of Populus deltoides, Populus trichocarpa, and hybrids of the two species. A panel of 102 Populus genotypes, comprising 37 genotypes of P. trichocarpa, 37 of P. deltoides and 28 unique hybrid genotypes (P. trichocarpa x P. deltoides and P. deltoides x P. trichocarpa) were evaluated in the greenhouse under two treatments, well-watered (WW) and drought (DS). Plant physiological data were collected throughout the experiment once the drought treatment began. Throughout the experiment, we tracked soil volumetric water content, pot weight, stomatal conductance, quantum yield of photosystem II, and electron transport rate. In addition to those measurements, upon completion of the experiment, we assessed above and belowground plant biomass, plant height and stem diameter, leaf number, specific leaf area, relative water content, total protein, and total chlorophyll. We obtained hyperspectral signatures of one leaf from each plant at the end of the experiment. Columns BC – LL are hyperspectral averages for one leaf from each plant at each wavelength as described in the column header.

本研究旨在鉴定美洲杨（Populus deltoides）、三角叶杨（Populus trichocarpa）及其种间杂交种的基因型间耐旱性差异。本试验共纳入102个杨属基因型，其中包含37个三角叶杨基因型、37个美洲杨基因型，以及28个独特的杂交种基因型（三角叶杨×美洲杨、美洲杨×三角叶杨）。试验在温室环境下设置两种处理：充分供水（well-watered, WW）与干旱胁迫（drought, DS）。干旱处理启动后，全程采集植株生理数据。试验期间，我们持续监测土壤体积含水量（soil volumetric water content）、盆钵重量、气孔导度（stomatal conductance）、光系统II量子产额（quantum yield of photosystem II）及电子传递速率（electron transport rate）。试验结束后，除上述测定指标外，我们还评估了植株地上与地下生物量（above and belowground plant biomass）、株高、茎粗、叶片数、比叶面积（specific leaf area）、相对含水量（relative water content）、总蛋白及总叶绿素含量。试验末期，我们采集了每株植物一片叶片的高光谱特征（hyperspectral signatures）数据。数据集的BC至LL列，为各波长下单叶的高光谱平均光谱值，具体信息见列标题说明。