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Figure S1 - Two Lysines in the Forkhead Domain of Foxp3 Are Key to T Regulatory Cell Function

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/Two_Lysines_in_the_Forkhead_Domain_of_Foxp3_Are_Key_to_T_Regulatory_Cell_Function/129718
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K17R and K18R did not inhibit the activity of NFAT1 on NFAT∶AP-1 site. 293T cells were transfected with NFAP1-luciferase vector, NFAT1 and WT Foxp3 or K17R or K18R. Forty hours later, cells were stimulated with 1 mM ionomycin, 10 nM PMA, and 2 mM CaCl2 for 6 hours, dual luciferase activity was measured and results normalized to renilla. (PPT)
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2015-12-02
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