Raw, trimmed, and mapped, small RNA seq reads

Raw and trimmed small RNA seq reads obtained from <em>Psylliodes chrysocephala </em>treated with double-stranded (ds) RNAs (dsSec23, dsVatpG, or dsGPF). The".bam" files are the output of Bowtie2, representing the coverage of reads perfectly mapping onto the respective dsRNA treatment. "_reads" files are the raw reads obtained by sequencing on a DNBSEQ-G400 platform and the "_trimmed.fq.gz" files represent the reads cleaned from adaptor sequences as well as reads having quality scores below 30 and lengths below 18 nt and above 25. The bam files could be investigated by, for instance, R package "viRome" which can output the coverages per position on the respective dsRNA sequence or directly using a software such as Tablet for which the dsRNA index files are required and also provided here. Of note the dsRNA sequence in the index file dsVatpG_index is antisense of its target gene whereas for dsSec23_index it is in sense direction. Another important note regarding the directionality is that all the raw reads are <br>

本数据集包含从经双链RNA（double-stranded RNA，dsRNA）（dsSec23、dsVatpG或dsGPF）处理的油菜蚤跳甲（<em>Psylliodes chrysocephala</em>）中获取的原始及经修剪的小RNA测序读段。其中，.bam格式文件为Bowtie2的输出结果，代表读段（reads）完美比对至对应dsRNA处理组的覆盖度。带"_reads"后缀的文件为在DNBSEQ-G400测序平台上获得的原始读段；带"_trimmed.fq.gz"后缀的文件则为经过接头序列去除、过滤掉质量值低于30、长度小于18 nt或大于25 nt的读段后得到的清洁读段。此类.bam文件可通过多种方式开展分析：例如使用R包"viRome"，该工具可输出对应dsRNA序列上每个位点的覆盖度；也可直接使用Tablet等可视化软件，但需提前准备并提供dsRNA索引文件。需注意的是，dsVatpG_index索引文件中的dsRNA序列与其靶基因序列为反义方向，而dsSec23_index索引文件中的dsRNA序列则与靶基因序列为正义方向。关于序列方向性的另一项重要说明为，所有原始读段均<br>