Transcriptome-wide impact of food-restriction on the medio-basal hypothalamus of the ewe.

Here we used RNAseq to compare the transcriptomes of ewes fed ad libitum VS food-restricted ewes (hereafter AL and RES, respectively). Body Condition Score was used as an index to validate the impact of food restriction. Overall design: The study was performed in adult ewes of the Ile-de-France breed. The experiment included 30 ewes in each group (ad lib and food-restricted) and was repeated the following year giving a grand total of 60 ewes fed ad lib and 60 food-restricted ewes. RNA was extracted from each ewe. Then, for each condition four random equimolar mixes of mRNA from 15 ewes were used for RNAseq --> 4 biological replicates.