Single-cell transcriptome sequencing of macrophages in acute kidney injury(AKI) to chronic kidney dieases(CKD)

Macrophages are key immune cells in AKI and may determine the fate of AKI to CKD progression. Here, by taking advantages of single cell RNA-sequencing technology, we generated a mononuclear macrophage atlas from the initiation of AKI till the progression to CKD. Overall design: We used a unilateral ischemia-reperfusion renal injury (uIRI) 45minAKI model. The mouse kidney, spleen, and peripheral blood were taken at different time points after surgery, and the kidney, spleen and peripheral blood of normal mice were used as controls. Mononuclear phagocytic cells were sorted by FACS and then performed single cell transcriptome sequencing. 6 mice at each time point.