Cell therapy in the treatment of bronchiolitis obliterans in a murine model

OBJECTIVE: To evaluate the importance of stem cells derived from adipose tissue in reducing graft inflammation in a murine model of allogeneic heterotopic tracheal transplant.METHODS: We performed a heterotopic tracheal allografting in dorsal subcutaneous pouch and systemically injected 5x10⁵ mesenchymal stem cells derived from adipose tissue. The animals were divided into two groups according to the time of sacrifice: T7 and T21. We also carried out histological analysis and digital morphometry.RESULTS: The T7 animals treated with cell therapy had median obstructed graft area of 0 versus 0.54 of controls (p = 0.635). The treated T21 subjects had median obstructed graft area of 0.25 versus 0 in controls (p = 0.041).CONCLUSION: The systemically injected cell therapy in experimental murine model of bronchiolitis obliterans did not reduce the severity of the allograft inflammation in a statistically significant way in seven days; Conversely, in 21 days, it increased the allograft inflammatory process.

研究目的：评估脂肪组织来源间充质干细胞（adipose tissue-derived mesenchymal stem cells）在同种异体异位气管移植（allogeneic heterotopic tracheal transplant）小鼠模型中减轻移植物炎症反应的作用。
研究方法：于小鼠背部皮下袋构建异位气管同种异体移植模型，并向其体内系统性注射5×10⁵个脂肪组织来源间充质干细胞。根据处死时间将实验动物分为两组：T7组与T21组。同时开展组织学分析与数字化形态计量学检测。
研究结果：接受细胞治疗的T7组小鼠移植物阻塞面积中位数为0，对照组为0.54（p=0.635）；接受细胞治疗的T21组小鼠移植物阻塞面积中位数为0.25，对照组为0（p=0.041）。
研究结论：在闭塞性细支气管炎（bronchiolitis obliterans）小鼠实验模型中，系统性注射细胞治疗在术后7天时未在统计学上显著减轻同种异体移植物的炎症反应严重程度；与之相反，术后21天时，该治疗反而加重了同种异体移植物的炎症进程。