Raw and normalized inhibitory activities for all nsp1 mutants.

The inhibitory functions for all nsp1 mutants are shown in this table expressed as averaged percent compared to nsp1-wt (set to 100 percent). Percent inhibitions were also normalized to expression levels. No values are reported for mutants nsp1-m8, -m25, and -m26 as they had no significant inhibitory activities and were expressed at a level too low to be measured. After expression normalization, many nsp1 mutants exhibited increased inhibitory abilities compared to nsp1-wt; for example, nsp1-m19 exhibits nearly four-fold more inhibitory functions over nsp1-wt. Even after normalization, nsp1-m16 continues to show a loss of inhibition of host IFN- and virus-dependent signaling while maintaining strong inhibition of host gene expression. (*indicates significant as compared to empty vector; #indicates significant as compared to nsp1-wt; significant is defined as a t-Test P-value ≤ 0.05).