INS-1E PBC
收藏Mendeley Data2024-01-31 更新2024-06-27 收录
下载链接:
https://data.caltech.edu/doi/10.22002/h4fpg-nwe24
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资源简介:
Tilt Series Date: 2018-07-15 Data Taken By: Stephen Carter Species / Specimen: Rat INS-1E pancreatic beta cells Strain: Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x. Microscope: Caltech Titan Krios Acquisition Software: SerialEM Upload Method: reupload_downloaded Processing Software Used: IMOD Collaborators and Roles: Ray Stevens Purification / Growth Conditions / Treatment: INS-1E cells grown on gold finder grids, stimulated with glucose. Sample Preparation: Manual Blotting
倾斜序列(Tilt Series)采集日期:2018-07-15
数据采集者:Stephen Carter
物种/样本:大鼠INS-1E胰腺β细胞(Rat INS-1E pancreatic beta cells)
品系(Strain):未标注
倾斜序列设置:单轴倾斜模式,倾斜范围为(-60.0°, 60.0°),步长1°,采用恒定角度增量,辐射剂量为120.0 eV/Ų,离焦量为-10.0 μm,放大倍数为34000x
显微镜:加州理工学院Titan Krios(Caltech Titan Krios)
采集软件:SerialEM
上传方式:reupload_downloaded
所用图像处理软件:IMOD
合作者及职责:Ray Stevens
纯化/培养条件/处理方式:INS-1E细胞培养于金质定位载网(gold finder grids)上,经葡萄糖刺激处理
样本制备:手动吸样(Manual Blotting)
创建时间:
2024-01-31



