Data from: Genome-wide analysis and endo-β-mannanase gene families expression profiling of tomato and soybean

Endo-β-mannanase is a type of cell wall degrading enzyme and it plays varied roles in different developmental events and/or different plants. Evolutionary analysis of the gene family will provide an insight into gene function and speciation. In this study, genome-wide analysis and expression profiling of tomato and soybean endo-β-mannanase gene families were performed. We have identified 11 and 20 endo-β-mannanase genes in the tomato and soybean genomes, respectively. Sequence alignment revealed three α-helices (αA, αB, and αC) of plant endo-β-mannanases were conserved in tomato, soybean and Arabidopsis. Gene structure and phylogenetic analysis implied that loss of introns play an important role in the evolution of endo-β-mannanases. Evolutionary analysis of endo-β-mannanases from various plant species showed that they diversified before the division of lower and higher plants. Promoter sequence analysis and expression patterns analysis found that endo-β-mannanases are not only involving in growth and development process of tomato, but also associated with the stress response in plants.

内切-β-甘露聚糖酶（endo-β-mannanase）是一类细胞壁降解酶，在不同发育事件及不同植物物种中发挥多样功能。对该基因家族开展进化分析，可为解析基因功能与物种形成机制提供重要见解。本研究针对番茄与大豆的内切-β-甘露聚糖酶基因家族进行了全基因组分析与表达谱研究。我们分别在番茄和大豆基因组中鉴定出11个和20个内切-β-甘露聚糖酶基因。序列比对结果显示，植物内切-β-甘露聚糖酶的3个α螺旋结构域（αA、αB与αC）在番茄、大豆及拟南芥中均具有保守性。基因结构与系统发育分析表明，内含子缺失在内切-β-甘露聚糖酶的进化过程中扮演关键角色。对多种植物物种的内切-β-甘露聚糖酶进行进化分析后发现，该类酶家族在低等植物与高等植物分化前就已发生多样化。启动子序列分析与表达模式分析结果证实，内切-β-甘露聚糖酶不仅参与番茄的生长发育进程，还与植物的胁迫响应过程密切相关。