Data associated with 'Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation'

Cryopreservation of mammalian cells has, to date, typically been conducted in cryovials, but there are applications where cryopreservation of primary cells in multiwell plates would be advantageous. However excessive supercooling in the small volumes of liquid in each well of the multiwell plates is inevitable without intervention and tends to result in high and variable cell mortality. We describe a technique for cryopreservation of adhered primary bovine granulosa cells in 96-well plates by controlled rate freezing using controlled nucleation. This dataset contains experimental data derived from granulosa cell culture cryopreservation experiments and measurements of ice nucleation temperature measurements observed within 96-well plates and other cryocontainers

迄今为止，哺乳动物细胞的冷冻保存通常以冷冻管（cryovials）为载体，但在部分应用场景中，采用多孔板（multiwell plates）保存原代细胞（primary cells）将更具优势。然而，若未施加干预措施，多孔板各孔内的微量液体会不可避免地发生过度过冷，进而导致细胞死亡率偏高且结果离散度较大。本研究报道了一种适用于96孔板内贴壁原代牛颗粒细胞（bovine granulosa cells）的冷冻保存方法，该方法通过可控成核（controlled nucleation）实现程序降温冻存（controlled rate freezing）。本数据集涵盖颗粒细胞培养冷冻保存实验产生的实验数据，以及在96孔板与其他冷冻容器（cryocontainers）中实测得到的冰核形成温度（ice nucleation temperature）数据。