IFN-lambda gut restitution scRNA-seq — merged WT + KO (20230801_WT_KO_Merge01): core analysis outputs

Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair To investigate the transcriptional program within the intestinal epithelial cells (IECs) controlling the impact of type III interferons (IFN-λ) on intestinal recovery, we performed a single-cell RNA sequencing (scRNA-seq) on murine IECs following irradiation. Mice deficient for the IFN-λ receptor in IECs and wild-type mice underwent targeted irradiation. 96h after irradiation, IECs were isolated by fluorescence-activated cell sorting (FACS) based on the expression of EPCAM and analyzed using scRNA-seq. This record Single archive core_Merge01.tar, 1854 files, 14.0 GB. Seurat objects (.RDS), rendered analysis reports (.html), reference files, container images and all light outputs of the pipeline. This record is self-contained: it reconstructs the full analysis tree for this dataset, without the regenerable heavy objects. How to use these records Every archive stores paths relative to the same root, 002_IFNL_Recovery_scRNAseq/. Download the archives you need and extract them all into one empty directory: tar xf *.tar Each file lands in its correct place in the project tree. Taking only the core records gives a complete but lightweight tree; adding rdata or bam records fills it in without overwriting anything.