Analysis of the peptide substrates identified from the IMAP substrate finder assay.
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The five peptides identified as substrates for CRK3:CYC6 were analysed by sequence alignment. The consensus sequence pattern follows the optimal recognition motif identified for mammalian CDKs.Sequence pattern: x S/T P x R/K.Optimal recognition motif for CDKs: x−1 (S/T0) P+1 x+2 (K/R+3).Underlined are the serine/threonine amino acid residues (in the 0 position) which are phosphorylated by the CRK3:CYC6 protein kinase complex.
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2015-12-02



