Plant growth analysis describing the soybean plants response on dryland field to seed co-inoculation

ABSTRACT: Plant growth analysis can be used for soybean plants evaluation to identify morphologic changes caused by soil microbes after seed inoculation. The objective was to measure changes of inoculated soybean plants grown under regular field Brazilian production conditions. The experiment was carried out to compare 5 inoculation treatments: T1 (Bradyrhizobium japonicum and B. diazoefficiens), T2 (T1 and Azospirillum brasilense), T3 (T1 and Trichoderma asperellum), T4 (T1 and T. virens, and Bacillus amyloliquefaciens) and T5 (T1 and Penicillum bilaiae). Leaf area, shoot and root dry matter were measured at vegetative and reproductive growth stages. Results of this study validate the contribution of plant growth analysis of soybeans plants for identifying their responses promoted by the combined inoculation of their seeds with selected microbes. Also, the microbial effects on plant growth vary not only between microbes but also between parts of the plants and through the plant development stages. The introduction of different microbes in soybean rhizosphere combined with Bradyrhizobium sp. strains can contribute to increase crop dry matter productivity during its growing cycle.

摘要：植物生长分析可用于大豆植株评价，以鉴定种子接种后土壤微生物诱导的形态变化。本研究旨在测定巴西常规田间生产条件下接种处理大豆植株的生长变化。实验设置5种接种处理组开展对照比较：T1（日本慢生根瘤菌（Bradyrhizobium japonicum）与狄氏慢生根瘤菌（B. diazoefficiens））、T2（T1与巴西固氮螺菌（Azospirillum brasilense））、T3（T1与棘孢木霉（Trichoderma asperellum））、T4（T1与绿木霉（T. virens）和解淀粉芽孢杆菌（Bacillus amyloliquefaciens））及T5（T1与拜莱青霉（Penicillum bilaiae））。于营养生长与生殖生长两个阶段，测定大豆的叶面积、地上部及根部干物质重量。本研究结果验证了大豆植物生长分析方法的应用价值，该方法可有效鉴定经筛选微生物复合种子接种后大豆的响应特征。此外，微生物对植物生长的调控效应不仅因微生物类群而异，还随植株部位及生长发育阶段动态变化。在大豆根际引入与慢生根瘤菌（Bradyrhizobium sp.）菌株复配的不同微生物类群，可在作物全生长周期内提升其干物质生产能力。