Single-cell RNA-seq analysis of human tumor ascites dendritic cells and macrophages

We performed single-cell RNA-seq on dendritic cells and macrophages isolated from the tumor ascites of one cancer patient. We used the 10x chromium technology. The expression matrix provided in supplementary is the result Cellranger aggregate method on : GSE115007, GSE115006 and GSE103544. Ascites phagocytes were prepared by centrifugation on a Ficoll gradient. Dendritic cells and macrophages were enriched by negative selection using magnetic beads. Cell populations were further isolated by cell sorting. Cellular suspensions (3500 cells) were loaded on a 10X Chromium instrument (10X Genomics) according to manufacturer’s protocol.