Pre-weaning heat stress alters liver transcriptome and DNA methylation in dairy calves | Laporta et al., JDS

This study investigated the impact of pre-weaning heat stress on dairy calves’ hepatic gene expression and DNA methylation. Holstein dairy calves were exposed to summer heat stress (daily average THI > 78) for 42 d postnatally (i.e., birth to weaning) with access to active fan heat abatement (postnatal cooling, post-CL; calf-height airspeed 2.05 m/s, n = 12) or not (postnatal heat stress, post-HS; calf-height airspeed 0.15 m/s, n = 12).
Liver tissue was harvested via biopsy (n = 6 per treatment) at 42 d of age for RNA sequencing and reduced representation bisulfite sequencing. The data sets in this repository contain the raw data for these analyses, including a list of differentially expressed genes and associated pathways and a list of differentially methylated genes and their related pathways and upstream regulators.

本研究探讨了断奶前热应激对奶牛犊肝基因表达和DNA甲基化的影响。荷斯坦奶牛犊在出生后42天内（即出生至断奶）暴露于夏季热应激（每日平均热应激指数THI超过78）中，并分为两组：一组有主动风扇降温处理（出生后冷却，后CL；犊牛高度风速2.05米/秒，n = 12），另一组则未进行任何处理（出生后热应激，后HS；犊牛高度风速0.15米/秒，n = 12）。在42日龄时，通过活检收集肝脏组织（每组n = 6）进行RNA测序和降低代表性亚硫酸氢盐测序。本存储库中的数据集包含这些分析的原始数据，包括差异表达基因及其相关通路列表，以及差异甲基化基因及其相关通路和上游调节因子列表。