Diverse ectomycorrhizal fungi communities found in urban reserve soils and scats of small mammals when compared to native forest

Ectomycorrhizal fungi (EMF) play an essential role in forest health. In urban reserves, there is a complex interplay between environmental factors, including anthropogenic stressors that influence the composition and structure of EMF communities, which in turn may affect their plant hosts and the presence of small mammals as dispersers. We aimed to assess the response of EMF to urbanisation by comparing the diversity and composition of their communities in small mammal scats and soil samples from urban reserves to those in native forest sites through ITS1 barcoding. Additionally, we analysed the effect of seasonality and the identity of small mammal vectors on EMF diversity present in scat samples. The datasets linked to our analyses include csv files with the identified EMF species and their abundance in samples of small mammal scats and soil. We found that EMF diversity was strongly influenced by the sample type (soil vs. scats). In soil samples, EMF communities in urban reserves were..., , # Diverse ectomycorrhizal fungi communities found in urban reserve soils and scats of small mammals when compared to native forest

Dataset DOI: [10.5061/dryad.jwstqjqns](10.5061/dryad.jwstqjqns)

## Description of the data and file structure

The files are in csv format and contain modified pre-curated and curated datasets used for the analyses. Soil and small mammal scat samples were collected in five forest sites and five urban reserves. The natural forest sites were located in Ku-ring-gai Chase National Park (KNP), whereas the five urban reserve sites were located around the boundary of KNP. For small mammal scat collection we sampled each pair of sites (forest and urban reserve) simultaneously for three consecutive nights. At each site, we installed 50 Elliot traps (33 cm x 8 cm x 9 cm) in a sampling grid spaced at 10 m intervals. We sampled the sites during both spring (September 2023) and autumn (April 2024) due to their contrasting rainfall patterns. Fresh scats were collected i...,

外生菌根真菌（Ectomycorrhizal fungi, EMF）在森林健康中发挥着不可或缺的作用。在城市保护区内，环境因素之间存在复杂的相互作用，包括影响外生菌根真菌群落组成与结构的人为胁迫因子，而这些真菌群落又会反过来影响其宿主植物以及作为传播者的小型哺乳动物的生存状况。本研究旨在通过ITS1条形码测序（ITS1 barcoding）技术，对比城市保护区与原生林中小型哺乳动物粪便及土壤样本中的外生菌根真菌群落多样性与组成，以此评估外生菌根真菌对城市化的响应。此外，本研究还分析了季节周期性以及小型哺乳动物传播媒介的种类对粪便样本中外生菌根真菌多样性的影响。本研究相关分析所用数据集包含csv格式文件，记录了已鉴定的外生菌根真菌物种及其在小型哺乳动物粪便与土壤样本中的丰度。研究发现，外生菌根真菌的多样性显著受样本类型（土壤vs粪便）影响。在土壤样本中，城市保护区的外生菌根真菌群落……
# 相较于原生林，城市保护区土壤与小型哺乳动物粪便中存在多样的外生菌根真菌群落
数据集DOI：[10.5061/dryad.jwstqjqns](10.5061/dryad.jwstqjqns)

## 数据与文件结构说明
所有文件均为csv格式，包含用于本研究分析的预处理及人工整理数据集。研究样本采集自5处原生林样地与5处城市保护区样地。原生林样地位于库林盖蔡斯国家公园（Ku-ring-gai Chase National Park, KNP），而5处城市保护区样地则分布于该国家公园的周边边界区域。小型哺乳动物粪便样本的采集采用配对样地同步采样方案，对每一组原生林-城市保护区配对样地连续三个夜间进行采样。在每个样地内，我们以10米为间隔布设采样网格，共放置50台埃利奥特捕鼠器（33 cm × 8 cm × 9 cm）。考虑到两个季节的降雨模式差异显著，我们分别于2023年9月（春季）与2024年4月（秋季）对样地进行了两次采样。新鲜粪便样本采集自……