Additional file 2 of Identification of a Major QTL and Candidate Gene Analysis of Salt Tolerance at the Bud Burst Stage in Rice (Oryza sativa L.) Using QTL-Seq and RNA-Seq
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Additional file 2: Table S1. An overview of the QTL-seq results. Table S2. SNPs results between two parents and two mixed pools. Table S3. Annotated genes within the interval detected by QTL-seq. Table S4. Phenotypic variation of parents and F2:3 lines. Table S5. Summary of Illumina transcriptome reads mapped to the reference genome. Table S6. Statistics of transcriptome sequencing results. Table S7. RNA-seq data of the genes in candidate region. Table S8. The KASP genotyping results. Table S9. ANOVA results of three genotypes. Table S10. The Arabidopsis homologous genes of Os07g0569700. Table S11. The genotype of 1 bp Indel in 66 diverse rice accessions. Table S12. Primers of 25 KASP markers. Table S13. The KASP reaction system. Table S14. Primers for qRT-PCR in this study.
附加文件2:表S1为QTL-seq(数量性状位点测序)结果概述。表S2为两个亲本与两个混合池间的单核苷酸多态性(Single Nucleotide Polymorphism, SNPs)结果。表S3为QTL-seq检测区间内的注释基因。表S4为亲本及F2:3家系的表型变异。表S5为比对至参考基因组的Illumina转录组读数汇总。表S6为转录组测序结果统计。表S7为候选区域内基因的RNA测序(RNA Sequencing, RNA-seq)数据。表S8为竞争性等位基因特异性PCR(Kompetitive Allele Specific PCR, KASP)基因分型结果。表S9为三种基因型的方差分析(Analysis of Variance, ANOVA)结果。表S10为Os07g0569700的拟南芥同源基因。表S11为66份不同水稻种质中1bp插入缺失(Insertion-Deletion, Indel)的基因型。表S12为25个KASP标记的引物序列。表S13为KASP反应体系。表S14为本研究中定量实时聚合酶链反应(quantitative Real-Time Polymerase Chain Reaction, qRT-PCR)所用引物。
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figshare
创建时间:
2020-08-11



